![]() ![]() Caution: Not intended for human or animal diagnostic or therapeutic uses. Note: The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards: supplemental data should be considered for more accurate adjustment in different electrophoresis conditions. Migration patterns of BLUelf prestained protein ladder in different electrophoresis conditions are listed below: G uide for Molecular Weight Estimation (kDa) Under suggested conditions, BLUelf prestained protein ladder resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after Western blotting to nitrocellulose membrane. Do not heat, dilute, add reducing agent before loading.Īpproximately 0.1-0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25☌), 2% SDS, 10 mM Dihiothreltol, 3.6 M Urea, and 15% (v/v) (Glycerol). The ladder is supplied in gel loading buffer and is ready to use. The BLUelf prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra prestained protein ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range molecular weights from 6.5 to 270 kDa. Apply more for thicker (>1.5mm) or larger gel. We offer a blue prestained protein standard, as well as a colored prestained protein standard with multi-colored. Sizes range from 10 to 250 kDa which is ideal for accurate molecular weight determination for a wide range of expressed proteins. 1.5-2.5μl per well for general Western transferring New England Biolabs offers a selection of highly pure protein standards. Malar J 19:367 (2020).- 3μl or 5μl per loading for clear visualization during electrophoresison 15-well or 10-well mini-gel, respectively. Population genetic and biophysical evidences reveal that purifying selection shapes the genetic landscape of Plasmodium falciparum RH ligands in Chhattisgarh and West Bengal, India. Non-synonymous amino acid alterations in PfEBA-175 modulate the merozoite ligand's ability to interact with host's Glycophorin A receptor. Group 1 metabotropic glutamate receptors trigger glutamate-induced intracellular Ca2+ signals and nitric oxide release in human brain microvascular endothelial cells. Effects of chemotherapy on placental development and function using in vitro culture of human primary cytotrophoblasts. PGRMC1 phosphorylation affects cell shape, motility, glycolysis, mitochondrial form and function, and tumor growth. Publishing research using ab116028? Please let us know so that we can cite the reference in this datasheet.Īb116028 has been referenced in 16 publications. Storage: Stable for up to 3 months at 4☌. This product was previously called Prism Ultra Protein Ladder (10-245 kDa). Review other protein ladders in the unstained and prestained protein ladder guide. Easy to identify: Includes green ~25 kDa and red ~75kDa reference bands.Ready-to-use: Supplied in a loading buffer for direct loading on gels.The protein ladder is supplied in gel loading buffer and is ready to use. This prestained protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer). Prestained Protein Ladder ab116028 is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. ![]()
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